Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold

in Combination with a Rotating Bioreactor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

157

Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi,

and Tiziana A. L. Brevini

Bioreactor-Based De-epithelialization of Long-Segment Tracheal Grafts . . . . . . . . . . .

167

Alba E. Marin-Araujo, Siba Haykal, and Golnaz Karoubi

Production of Extracellular Vesicles Using a CELLine Adherent

Bioreactor Flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

183

Anastasiia Artuyants, Vanessa Chang, Gabrielle Reshef,

Cherie Blenkiron, Lawrence W. Chamley, Euphemia Leung,

and Colin L. Hisey

Extracellular Vesicle Collection from Human Stem Cells Grown

in Suspension Bioreactors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

193

Xuegang Yuan, Xingchi Chen, Changchun Zeng, David G. Meckes Jr,

and Yan Li

Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies:

An In Vitro Bioreactor Perfusion Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

205

Max Wacker, Jan Riedel, Priya Veluswamy, Maximilian Scherner,

Jens Wippermann, Heike Walles, and Jo¨rn Hu¨lsmann

A Guideline to Set Up Cascaded Continuous Cultivation

with E. coli Bl21 (DE3) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

223

Julian Kopp and Oliver Spadiut

Applying Stirred Perfusion to 3D Tissue Equivalents to Mimic

the Dynamic In Vivo Microenvironment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

241

Henry W. Hoyle, Claire L. Mobbs, and Stefan A. Przyborski

Bioreactor-Based Adherent Cells Harvesting from Microcarriers

with 3D Printed Inertial Microfluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

257

Lin Ding, Reza Moloudi, and Majid Ebrahimi Warkiani

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

267

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